CAS No. 305-03-3

Batch: Purity:99.174
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Chloroambucil Chemical Structure
Chemical Structure
Check the Chloroambucil product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[4-[bis(2-chloroethyl)amino]phenyl]butanoic acid
CAS Number 305-03-3
Molecular Formula

C14H19Cl2NO2
Molecular Weight (MW) 304.21
SMILES C1=CC(=CC=C1CCCC(=O)O)N(CCCl)CCCl
InChIKey JCKYGMPEJWAADB-UHFFFAOYSA-N

Ordering Information

Biological Activity

Chloroambucil is a nitrogen mustard alkylating agent that functions by forming DNA interstrand cross-links. This persistent DNA damage inhibits replication and transcription, triggering activation of the p38 MAPK and JNK signaling pathways. Downstream cellular effects include caspase activation, PARP cleavage, and cell cycle arrest, ultimately leading to selective apoptotic cell death in B-cell chronic lymphocytic leukemia cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 61 mg/mL (200.51 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 61 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.