CAS No. 3697-42-5

Batch: Purity:99.75
Chlorhexidine 2HCl Chemical Structure
Chemical Structure
Check the Chlorhexidine 2HCl product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 3697-42-5
Molecular Formula

C22H30Cl2N10.2HCl

Molecular Weight (MW) 578.37
SMILES C1=CC(=CC=C1NC(=NC(=NCCCCCCN=C(N)N=C(N)NC2=CC=C(C=C2)Cl)N)N)Cl.Cl.Cl

Ordering Information

Biological Activity

Chlorhexidine 2HCl is a broad-spectrum bisbiguanide antiseptic active against gram-positive and gram-negative bacteria. It acts by binding to negatively charged bacterial cell wall components, inducing cell membrane disruption and cytoplasmic leakage. At effective concentrations, this membrane damage causes precipitation of intracellular proteins and nucleic acids, ultimately leading to bacterial cell death. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (138.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.