CAS No. 2610-05-1

Batch: Purity:98
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Chicago Sky Blue 6B Chemical Structure
Chemical Structure
Check the Chicago Sky Blue 6B product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 2610-05-1
Molecular Formula

C34H24N6Na4O16S4
Molecular Weight (MW) 992.8
SMILES COC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)OC)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+]

Ordering Information

Biological Activity

Chicago Sky Blue 6B is a small-molecule inhibitor of macrophage migration inhibitory factor (MIF) and vesicular glutamate transport, as well as a counterstain used to reduce background autofluorescence in immunofluorescence histochemistry. By blocking vesicular glutamate uptake, it alters glutamatergic neurotransmission and suppresses downstream inflammatory signaling pathways. These actions attenuate background fluorescence signals in tissue imaging and modulate cellular phenotypes associated with inflammatory and neurochemical responses. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 20 mg/mL (20.14 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.