CAS No. 153-61-7

Batch: Purity:99.8
Cephalotin acid Chemical Structure
Chemical Structure
Check the Cephalotin acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 153-61-7
Molecular Formula

C16H16N2O6S2

Molecular Weight (MW) 396.44
SMILES CC(=O)OCC1=C(N2C(C(C2=O)NC(=O)CC3=CC=CS3)SC1)C(=O)O

Ordering Information

Biological Activity

Cephalotin acid (Cephalothin) is a broad-spectrum, first-generation cephalosporin antibiotic that targets penicillin-binding proteins (PBPs) involved in bacterial cell wall peptidoglycan synthesis. By irreversibly inhibiting PBP-mediated transpeptidation, it disrupts cell wall structural integrity, leading to osmotic instability, cell wall degradation, and bacterial cell lysis. Cephalothin demonstrates potent bactericidal activity against a wide range of Gram-positive and select Gram-negative bacterial pathogens.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 79 mg/mL (199.27 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.