CAS No. 23325-78-2

Batch: Purity:99.09
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Cephalexin Monohydrate Chemical Structure
Chemical Structure
Check the Cephalexin Monohydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 23325-78-2
Molecular Formula

C16H19N3O5S
Molecular Weight (MW) 365.4
SMILES CC1=C(N2C(C(C2=O)NC(=O)C(C3=CC=CC=C3)N)SC1)C(=O)O.O

Biological Activity

Cephalexin Monohydrate is a first-generation cephalosporin antibiotic that targets bacterial penicillin-binding proteins, particularly penicillin-binding protein 3. By inhibiting penicillin-binding proteins, it blocks the final transpeptidation step of peptidoglycan synthesis required for cell wall assembly. This disruption leads to morphological alterations, structural integrity loss, cell filamentation, and ultimate bactericidal cell lysis in susceptible bacterial strains. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
4-Methylpyridine 14 mg/mL
DMSO 0.01 mg/mL (0.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Spratt BG. Proc Natl Acad Sci U S A. 1975;72(8):2999-3003.

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Note: Technical data last updated: Sep 1, 2026.