CAS No. 95789-30-3

Batch: Purity:97.96
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Cefotiam Hexetil Hydrochloride Chemical Structure
Chemical Structure
Check the Cefotiam Hexetil Hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 95789-30-3
Molecular Formula

C27H39Cl2N9O7S3
Molecular Weight (MW) 768.76
SMILES CC(OC(=O)C1=C(CSC2N1C(=O)C2NC(=O)CC3=CSC(=N3)N)CSC4=NN=NN4CCN(C)C)OC(=O)OC5CCCCC5.Cl.Cl

Biological Activity

Cefotiam Hexetil Hydrochloride is an orally active prodrug of cefotiam, a semi-synthetic beta-lactam cephalosporin antibiotic that targets bacterial penicillin-binding proteins. By binding to these enzymes, it interferes with the transpeptidation process during peptidoglycan cell wall synthesis. This inhibition weakens bacterial cell wall structural integrity, triggering osmotic lysis and ultimate cell death in susceptible bacteria. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (130.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 33 mg/mL
Ethanol 22 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Spratt BG. Eur J Biochem. 1977;72(2):341-52.

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Note: Technical data last updated: Sep 1, 2026.