CAS No. 22252-43-3

Batch: Purity:98
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Cefalexine EP IMpurity B Chemical Structure
Chemical Structure
Check the Cefalexine EP IMpurity B product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 22252-43-3
Molecular Formula

C8H10N2O3S
Molecular Weight (MW) 214.24
SMILES CC1=C(N2C(C(C2=O)N)SC1)C(=O)O

Biological Activity

Cefalexine EP Impurity B (7-aminodesacetoxycephalosporanic acid, 7-ADCA) is a key beta-lactam core derivative and essential precursor used in the bioconversion and enzymatic synthesis of semi-synthetic cephalosporin antibiotics, including cephalexin. Serving as a primary nucleus substrate in biocatalytic acylation reactions mediated by enzymes such as penicillin acylase, it enables the formation of active cephalosporins that block bacterial cell wall peptidoglycan cross-linking to inhibit cell growth.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
5%TFA 3.03 mg/mL
DMSO 0.01 mg/mL (0.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.