CAS No. 225120-65-0

Batch: Purity:99.87
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Cathepsin Inhibitor 1 Chemical Structure
Chemical Structure
Check the Cathepsin Inhibitor 1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3-tert-butyl-N-[(2S)-3-(3-chlorophenyl)-1-(cyanomethylamino)-1-oxopropan-2-yl]-1-methylpyrazole-5-carboxamide
CAS Number 225120-65-0
Molecular Formula

C20H24ClN5O2
Molecular Weight (MW) 401.89
SMILES CC(C)(C)C1=NN(C(=C1)C(=O)NC(CC2=CC(=CC=C2)Cl)C(=O)NCC#N)C
InChIKey MZRVIHRERYCHBL-HNNXBMFYSA-N

Biological Activity

Cathepsin Inhibitor 1 is a potent inhibitor of multiple cysteine cathepsins, targeting cathepsin L, cathepsin L2, cathepsin S, cathepsin K, and cathepsin B. By inhibiting these lysosomal cysteine proteases, the compound disrupts intracellular protein degradation, extracellular matrix remodeling, and antigen processing. This pathway suppression attenuates tumor cell invasion, extracellular matrix degradation, and downstream inflammatory signaling cascades. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (199.05 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 50 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Mohamed MM, et al. Nat Rev Cancer. 2006;6(10):764-75.

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Note: Technical data last updated: Sep 1, 2026.