Carbamoylcholine chloride (Carbachol)
For research use only.
Catalog No.S4359 Synonyms: Carbamylcholine chloride, Carbastat, Miostat
CAS No. 51-83-2
Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors.
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|Description||Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors.|
Carbachol causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. Carbachol increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers.  Carbachol (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. Carbachol (100 μM) stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Carbachol (100 μM) stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells.  Carbachol (3.3 nmol dose) produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. Carbachol causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats. 
Carbachol (1-10 ng) infused specifically into the posterior oral pontine reticular nucleus (PnO) results in induction of rapid eye movement sleep in the rat. Carbachol (500 ng) does not affect REM sleep but enhances wakefulness in the rat. 
-  Dharmsathaphorn K, et al. J Clin Invest, 1986, 77(2), 348-354.
-  Keely SJ, et al. J Biol Chem, 1998, 273(42), 27111-27117.
-  French SJ, et al. Brain Res Mol Brain Res, 1999, 67(1), 124-136.
|In vitro||Water||36 mg/mL (197.09 mM)|
|DMSO||5 mg/mL warmed (27.37 mM)|
|Ethanol||''12 mg/mL warmed|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||Carbamylcholine chloride, Carbastat, Miostat|
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
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|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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