Home Neuronal Signaling AChR agonist Carbamoylcholine chloride (Carbachol)

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Carbamoylcholine chloride (Carbachol) AChR agonist

Cat.No.S4359

Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Carbamoylcholine chloride (Carbachol) AChR agonist Chemical Structure

Chemical Structure

Molecular Weight: 182.65

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Quality Control

Batch: Purity: 98.89%
98.89

Solubility

In vitro
Batch:

Water : 36 mg/mL

Ethanol : 12 mg/mL

DMSO : 5 mg/mL (27.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

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In vivo
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Chemical Information, Storage & Stability

Molecular Weight 182.65 Formula

C6H15N2O2.Cl

 Storage (From the date of receipt)
CAS No. 51-83-2 Download SDF Storage of Stock Solutions

Synonyms Carbamylcholine chloride, Carbastat, Miostat SMILES [Cl-].C[N+](C)(C)CCOC(N)=O

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Mechanism of Action

In vitro

Carbamoylcholine chloride (Carbachol) causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. It increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers. This compound (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. It also stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Additionally, it stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells. At a 3.3 nmol dose, it produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. It also causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats.

In vivo

Infused specifically into the posterior oral pontine reticular nucleus (PnO) of the rat, Carbamoylcholine chloride (Carbachol) (1–10 ng) induces rapid eye movement sleep. At 500 ng, it does not affect REM sleep but enhances wakefulness.

References
  • [4] https://pubmed.ncbi.nlm.nih.gov/7766858/
  • [5] https://pubmed.ncbi.nlm.nih.gov/15817635/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-04-29)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06948357 RECRUITING
Presbyopia
Zhaoke (Guangzhou) Ophthalmology Pharmaceutical Ltd.
 2025-03-24 PHASE2
NCT05135286 COMPLETED
Presbyopia
Visus Therapeutics
 2022-03-15 PHASE3
NCT05270863 COMPLETED
Presbyopia
Visus Therapeutics
 2022-03-30 PHASE3
NCT04774237 COMPLETED
Presbyopia
Visus Therapeutics
 2021-03-24 PHASE2
NCT05225597 COMPLETED
Epinephrine Toxicity; Cataract; Carbachol Adverse Reaction; Choroid Disease
Ordu University
 2021-01-01
NCT00564408 COMPLETED
Headache
Danish Headache Center
 2007-08

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