Carbamoylcholine chloride (Carbachol)

For research use only.

Catalog No.S4359 Synonyms: Carbamylcholine chloride, Carbastat, Miostat

Carbamoylcholine chloride (Carbachol) Chemical Structure

CAS No. 51-83-2

Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors.

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Biological Activity

Description Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors.
In vitro

Carbachol causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. Carbachol increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers. [1] Carbachol (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. Carbachol (100 μM) stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Carbachol (100 μM) stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells. [2] Carbachol (3.3 nmol dose) produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. Carbachol causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats. [3]

In vivo

Carbachol (1-10 ng) infused specifically into the posterior oral pontine reticular nucleus (PnO) results in induction of rapid eye movement sleep in the rat. Carbachol (500 ng) does not affect REM sleep but enhances wakefulness in the rat. [4]

Protocol

Solubility (25°C)

In vitro Water 36 mg/mL (197.09 mM)
DMSO 5 mg/mL warmed (27.37 mM)
Ethanol ''12 mg/mL warmed

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Chemical Information

Molecular Weight 182.65
Formula

C6H15N2O2.Cl

CAS No. 51-83-2
Storage powder
in solvent
Synonyms Carbamylcholine chloride, Carbastat, Miostat
Smiles [Cl-].C[N+](C)(C)CCOC(N)=O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID