Cell-free Assays (IC50/Ki) for Buparlisib (BKM120)

Key TargetIC50 (Cell-free)DescriptionReference
PI3K α 44.6 ± 3.6 nM In vitro inhibition assay Download PDF
PI3K β 279.0 ± 19.2 μM In vitro inhibition assay Download PDF
PI3K γ 192.8 ± 23.3 μM In vitro inhibition assay Download PDF
PI3K δ 79.3 ± 11.0 nM In vitro inhibition assay Download PDF
mTOR >10,000 μM In vitro inhibition assay Download PDF

Cell-based Assay (IC50) for Buparlisib (BKM120)

Cell TypeIC50 (Cell-based)DescriptionReference
A549 63.9 μM MTT assays PMC6643147
H661 63.9 μM MTT assays PMC6643147
SKLMS1 800.0 nM ATPlite assay, fluorescence read on a PerkinElmer Enspire 2300 multimode plate reader, EC50 curves generated and analysed using GraphPad Prism 4.0. Download PDF
STS39 513.2 nM ATPlite assay, fluorescence read on a PerkinElmer Enspire 2300 multimode plate reader, EC50 curves generated and analysed using GraphPad Prism 4.0. Download PDF
BCR-ABL negative ALL-LTCs 0.5µM XTT assay (proliferation) Download PDF
Check the Buparlisib (BKM120) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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