CAS No. 2760881-74-9

Batch: Purity:99.56
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BRD0639 Chemical Structure
Chemical Structure
Check the BRD0639 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-2-(4-chloro-6-oxopyridazin-1-yl)-N-[4-methyl-3-(2-pyridin-2-ylethylsulfamoyl)phenyl]propanamide
CAS Number 2760881-74-9
Molecular Formula

C21H22ClN5O4S
Molecular Weight (MW) 475.948
SMILES CC(N1N=CC(=CC1=O)Cl)C(=O)NC2=CC=C(C)C(=C2)[S](=O)(=O)NCCC3=NC=CC=C3
InChIKey BXDCFRRDENJUJM-HNNXBMFYSA-N

Biological Activity

BRD0639 is a first-in-class, PRMT5 binding motif (PBM)-competitive small-molecule inhibitor that disrupts the interaction between PRMT5 and its substrate adaptor protein RIOK1. By forming a covalent bond with cysteine 278 of PRMT5, BRD0639 inhibits substrate adaptor binding and blocks arginine methylation of target proteins, leading to reduced PBM-dependent PRMT5 methyltransferase activity in cellular models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 95 mg/mL (199.6 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 95 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] McKinney DC, et al. J Med Chem. 2021;64(15):11148-11168.

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Note: Technical data last updated: Sep 1, 2026.