CAS No. 121207-31-6
Batch:
Purity:98.69
Chemical Structure
Check the
BODIPY 493/503
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 2,2-difluoro-4,6,8,10,12-pentamethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene | |
|---|---|---|
| CAS Number | 121207-31-6 | |
| Molecular Formula |
C14H17BF2N2 |
|
| Molecular Weight (MW) | 262.11 | |
| InChIKey | DRJHPEGNOPSARR-UHFFFAOYSA-N |
Biological Activity
BODIPY 493/503 is a cell-permeable, fluorescent neutral lipophilic dye that selectively targets intracellular neutral lipids and lipid droplets. Upon partitioning into non-polar lipid core structures, the dye exhibits enhanced green fluorescence, enabling precise visualization and quantification of lipid droplet accumulation by fluorescence microscopy and flow cytometry. This fluorophore facilitates cellular lipid metabolism research, including investigations into lipid droplet biogenesis, lipolysis, and lipotoxicity under various metabolic conditions. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
4 mg/mL
(15.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.