Home Others Other BM213 For research use only.

BM213

Synonyms:

BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.

BM213 Chemical Structure

BM213 Chemical Structure

CAS:

Purity & Quality Control

Batch: P121501 Water] 100 mg/mL] false] ] ] false] ] ] false Purity: 98%
98

BM213 Related Products

Biological Activity

Description BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.
In vitro
In vitro

BM213 is a potent and stable peptide C5aR1 agonists that display no C5aR2 activity and over 1000-fold selectivity for C5aR1 over C3aR. BM213 induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is functionally similar to C5a in human macrophage cytokine release assays and in a murine in vivo neutrophil mobilization assay.[1]
Cell Research Cell lines HMDMs, SHSY-5Y cells
Concentrations 0.1-100 μM
Incubation Time 0-6 h, 24 h
Method

HMDMs are stimulated with LPS (10 ng/mL) alone or in the presence of BM213. The supernatant content of cytokines after 24 h stimulation is quantified using ELISA. For MTT cell survival assay, differentiated SH-SY5Y cells are treated with varying concentrations of BM213 following a 2.5 h incubation.
In Vivo
In vivo

BM213 shows antitumor activity in a mouse model of mammary carcinoma.[1]
Animal Research Animal Models Mammary carcinoma model mice bearing with EMT-6 cells
Dosages 1mg/kg
Administration IOCV

Chemical Information & Solubility

Molecular Weight Formula
CAS No. SDF --
Density 95.0~105.0%
Storage (From the date of receipt) 3 years-20°C powder

In vitro
Batch:

Water : 100 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: ≤1.0%