CAS No. 23491-45-4

Batch: Purity:99.60
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bisBenzimide H 33258 Trihydrochloride Chemical Structure
Chemical Structure
Check the bisBenzimide H 33258 Trihydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 23491-45-4
Molecular Formula

C25H24N6O.3ClH
Molecular Weight (MW) 533.88

Biological Activity

bisBenzimide H 33258 Trihydrochloride is a cell-permeable bisbenzimide fluorescent dye that selectively targets adenine-thymine-rich minor grooves of double-stranded DNA. Binding to double-stranded DNA significantly enhances its fluorescence intensity, making it widely used for live-cell nuclear labeling and chromatin visualization. Downstream interaction with cellular DNA modulates topoisomerase activity, resulting in inhibited DNA replication and antiproliferative effects against tumor cells.[1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 31 mg/mL (58.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 23 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Pjura PE, et al. J Mol Biol. 1987;197(2):257-271.

[2] McHugh MM, et al. Biochem Pharmacol. 1989;38(14):2323-2328.

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Note: Technical data last updated: Sep 1, 2026.