CAS No. 35013-72-0
Batch:
Purity:100.00
Chemical Structure
Check the
(+)-Biotin N-hydroxysuccinimide ester
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 35013-72-0 | |
|---|---|---|
| Molecular Formula |
C14H19N3O5S |
|
| Molecular Weight (MW) | 341.38 |
(+)-Biotin N-hydroxysuccinimide ester is an amine-reactive biotinylation reagent that selectively acylates primary amino groups on peptides, proteins, and cell surface molecules. By forming stable amide bonds, this reagent enables covalent conjugation of biotin tags to biological targets, facilitating downstream affinity purification, detection, and cell surface labeling via avidin or streptavidin interaction without disrupting target function or cell viability.[1][2]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
68 mg/mL
(199.19 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
- (+)-Biotin N-hydroxysuccinimide ester Solubility in DMSO
- (+)-Biotin N-hydroxysuccinimide ester Stock Solution
- (+)-Biotin N-hydroxysuccinimide ester Storage
- (+)-Biotin N-hydroxysuccinimide ester Stability
- (+)-Biotin N-hydroxysuccinimide ester Molecular Weight
- (+)-Biotin N-hydroxysuccinimide ester Chemical Structure (2D and 3D)
- (+)-Biotin N-hydroxysuccinimide ester SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.