CAS No. 150-25-4

Batch: Purity:99.04
Check CoA
Bicine Chemical Structure
Chemical Structure
Check the Bicine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 150-25-4
Molecular Formula

C6H13NO4
Molecular Weight (MW) 163.17
SMILES C(CO)N(CCO)CC(=O)O

Ordering Information

Biological Activity

Bicine is a zwitterionic organic buffering agent widely utilized in biochemical and molecular biology applications. Operating effectively within a pH range of 7.6 to 9.0, it stabilizes physiological pH during enzymatic reactions, protein purification, and electrophoresis. By maintaining optimal ionic strength and pH stability without interfering with most biological processes, Bicine supports downstream biochemical assays, enzyme activity assays, and native protein separation techniques.

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 33 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.