CAS No. 1227948-02-8

Batch: Purity:99.63
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BI-4464 Chemical Structure
Chemical Structure
Check the BI-4464 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3-methoxy-N-(1-methylpiperidin-4-yl)-4-[[4-[(3-oxo-1,2-dihydroinden-4-yl)oxy]-5-(trifluoromethyl)pyrimidin-2-yl]amino]benzamide
CAS Number 1227948-02-8
Molecular Formula

C28H28F3N5O4
Molecular Weight (MW) 555.55
SMILES CN1CCC(CC1)NC(=O)C2=CC(=C(C=C2)NC3=NC=C(C(=N3)OC4=CC=CC5=C4C(=O)CC5)C(F)(F)F)OC
InChIKey QUSSZSMDFABHLI-UHFFFAOYSA-N

Biological Activity

BI-4464 is a highly selective, ATP-competitive inhibitor of focal adhesion kinase (PTK2/FAK). By targeting PTK2/FAK, BI-4464 disrupts integrin-mediated signal transduction and focal adhesion dynamics. Inhibition of this kinase pathway impairs downstream signaling essential for cell adhesion, migration, invasion, and survival, leading to suppressed tumor cell motility and altered cytoskeletal organization in preclinical cancer models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 25 mg/mL (45.0 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 13 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Sulzmaier FJ, et al. Nat Rev Cancer. 2014;14(9):598-610.

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Note: Technical data last updated: Sep 1, 2026.