CAS No. 2894848-07-6
Batch:
Purity:99.02
Chemical Structure
Check the
BI-3231
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1-[[5-(2,4-difluoro-3-hydroxyphenyl)-1,3,4-thiadiazol-2-yl]methyl]-3-ethyl-5-methylpyrimidine-2,4-dione | |
|---|---|---|
| CAS Number | 2894848-07-6 | |
| Molecular Formula |
C16H14F2N4O3S |
|
| Molecular Weight (MW) | 380.37 | |
| InChIKey | XKDHFIPNTTUSIA-UHFFFAOYSA-N |
Biological Activity
BI-3231 is a potent and selective chemical probe that inhibits hydroxysteroid 17β-dehydrogenase 13 (HSD17B13) with an ICof 1 nM and a Ki ofin enzymatic assays. By targeted inhibition of HSD17B13, BI-3231 modulates hepatic lipid metabolism, attenuating intracellular triglyceride accumulation and reducing lipotoxicity in hepatocytes. These effects contribute to improved hepatocyte proliferation and lipid homeostasis, highlighting its therapeutic potential for nonalcoholic steatohepatitis and related liver diseases. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
76 mg/mL
(199.8 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.