CAS No. 2375482-51-0

Batch: Purity:99.87
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BI-2852 Chemical Structure
Chemical Structure
Check the BI-2852 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (3S)-5-hydroxy-3-[2-[[[1-[(1-methylimidazol-4-yl)methyl]indol-6-yl]methylamino]methyl]-1H-indol-3-yl]-2,3-dihydroisoindol-1-one
CAS Number 2375482-51-0
Molecular Formula

C31H28N6O2
Molecular Weight (MW) 516.59
SMILES CN1C=C(N=C1)CN2C=CC3=C2C=C(C=C3)CNCC4=C(C5=CC=CC=C5N4)C6C7=C(C=CC(=C7)O)C(=O)N6
InChIKey JYEQLXOWWLNVDX-PMERELPUSA-N

Biological Activity

BI-2852 is a potent KRAS inhibitor that binds with nanomolar affinity to the pocket between switch I and switch II on RAS. By occupying this switch pocket, BI-2852 blocks GEF, GAP, and effector interactions with KRAS, thereby suppressing downstream MAPK and AKT signaling pathways to induce antiproliferative effects in the low micromolar range in KRAS-mutant cell lines.[1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (193.57 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Kessler D, et al. Proc Natl Acad Sci U S A. 2019;116(32):15823-15829.

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Note: Technical data last updated: Sep 1, 2026.