CAS No. 141388-76-3

Batch: Purity:98.76
Besifloxacin Chemical Structure
Chemical Structure
Check the Besifloxacin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 141388-76-3
Molecular Formula

C19H21ClFN3O3

Molecular Weight (MW) 393.84
SMILES C1CCN(CC(C1)N)C2=C(C=C3C(=C2Cl)N(C=C(C3=O)C(=O)O)C4CC4)F

Ordering Information

Biological Activity

Besifloxacin is a fourth-generation fluoroquinolone antibiotic that functions as a dual inhibitor of bacterial DNA gyrase and topoisomerase IV. By targeting these essential type II topoisomerases, it inhibits bacterial DNA replication, recombination, and repair processes. This inhibition leads to the accumulation of double-stranded DNA breaks, ultimately causing cell death across a broad spectrum of Gram-positive and Gram-negative bacterial pathogens. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
tert-Butanol 3 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.