CAS No. 405165-61-9
Batch:
Purity:99.02
Chemical Structure
Check the
Besifloxacin HCl
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 405165-61-9 | |
|---|---|---|
| Molecular Formula |
C19H21ClFN3O3.HCl |
|
| Molecular Weight (MW) | 430.3 | |
| SMILES | C1CCN(CC(C1)N)C2=C(C=C3C(=C2Cl)N(C=C(C3=O)C(=O)O)C4CC4)F.Cl |
Biological Activity
Besifloxacin HCl is a fourth-generation fluoroquinolone antibiotic that functions as a dual inhibitor of bacterial DNA gyrase and topoisomerase IV. By targeting these essential type II topoisomerases, it disrupts bacterial DNA replication, transcription, repair, and recombination. This inhibition leads to the accumulation of double-stranded DNA breaks and ultimately induces rapid bacterial cell death across a broad spectrum of Gram-positive and Gram-negative pathogens. [1]
How to Prepare Stock Solution?
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Concentration
Volume
Mass
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|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| Water | 1 mg/mL |
| DMSO |
Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.) |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
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Note: Technical data last updated: Sep 1, 2026.