CAS No. 161804-20-2

Batch: Purity:99.31
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Benzylamiloride hydrochloride Chemical Structure
Chemical Structure
Check the Benzylamiloride hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide;hydrochloride
CAS Number 161804-20-2
Molecular Formula

C13H14ClN7O.ClH
Molecular Weight (MW) 356.21
SMILES C1=CC=C(C=C1)CN=C(N)NC(=O)C2=C(N=C(C(=N2)Cl)N)N.Cl
InChIKey ZNWMRWWNJBXNKJ-UHFFFAOYSA-N

Ordering Information

Biological Activity

Benzylamiloride hydrochloride is a potent inhibitor of the Na+/Ca2+ exchanger (NCX) with an IC50 of approximately. By blocking cellular sodium influx, it regulates intracellular ion homeostasis and prevents pathological sodium overload. In vivo, this functional blockade significantly reduces hyponatremic brain edema, protecting neural tissue against osmotic strain and swelling. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 98 mg/mL (275.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 3 mg/mL
Water 2 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.