CAS No. 2254805-44-0
Batch:
Purity:99.80
Chemical Structure
Check the
BBT-176
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | N-[2-[[5-chloro-2-[2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]anilino]pyrimidin-4-yl]amino]phenyl]methanesulfonamide | |
|---|---|---|
| CAS Number | 2254805-44-0 | |
| Molecular Formula |
C28H37ClN8O3S |
|
| Molecular Weight (MW) | 601.17 | |
| InChIKey | MPISIZFSGAZROV-UHFFFAOYSA-N |
Biological Activity
BBT-176 is a reversible, fourth-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor designed to target osimertinib-resistant mutations. It potently inhibits EGFR harboring C797S-containing triple and double resistance mutations, including Del19/T790M/C797S and L858R/T790M/C797S. By blocking EGFR phosphorylation, BBT-176 suppresses downstream MAPK/ERK and PI3K/AKT signaling pathways, thereby inhibiting cell proliferation and inducing apoptosis in resistant non-small cell lung cancer models. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
50 mg/mL
(83.17 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.