CAS No. 3567-69-9

Batch: Purity:99.26
Azo Rubine Chemical Structure
Chemical Structure
Check the Azo Rubine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 3567-69-9
Molecular Formula

C20H12N2Na2O7S2

Molecular Weight (MW) 502.43
SMILES C1=CC=C2C(=C1)C(=CC=C2S(=O)(=O)[O-])N=NC3=C(C4=CC=CC=C4C(=C3)S(=O)(=O)[O-])O.[Na+].[Na+]

Ordering Information

Biological Activity

Azo Rubine is a synthetic azo dye that acts as a substrate for bacterial and hepatic azoreductase enzymes. Metabolism of Azo Rubine via azoreductase-mediated cleavage of the azo linkage generates aromatic amine metabolites, modulating cellular redox balance and downstream signaling pathways. In vitro characterization shows that Azo Rubine binds to human serum albumin primarily through hydrophobic interactions and electrostatic forces to mediate systemic transport.

How to Prepare Stock Solution?

Concentration Volume Mass
1 mg 5 mg 10 mg
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (199.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.