CAS No. 320-67-2
| IUPAC Name | 4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,3,5-triazin-2-one | |
|---|---|---|
| CAS Number | 320-67-2 | |
| Molecular Formula |
C8H12N4O5 |
|
| Molecular Weight (MW) | 244.2 | |
| SMILES | C1=NC(=NC(=O)N1C2C(C(C(O2)CO)O)O)N | |
| InChIKey | NMUSYJAQQFHJEW-KVTDHHQDSA-N |
5-Azacytidine is a cytidine nucleoside analogue that acts as a DNA methyltransferase (DNMT) inhibitor. Upon incorporation into nucleic acids during replication and transcription, it traps DNA methyltransferases into stable covalent complexes, resulting in enzyme depletion and concentration-dependent DNA demethylation. This loss of methylation restores silenced gene expression, disrupts cellular regulation, and ultimately triggers cellular responses such as mitochondrial apoptosis and autophagy in treated cancer cell lines. [1][2]
|
Concentration
Volume
Mass
|
|---|
| Solvent | Solubility & Note |
|---|---|
| DMSO |
48 mg/mL
(196.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
49 mg/mL
(200.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
48 mg/mL
(196.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
48 mg/mL
(196.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| Water | 50 mg/mL (Ultrasonic heating for 5 minutes) |
| DMSO |
48 mg/mL
(196.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | Insoluble |
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.
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