CAS No. 1421373-98-9

Batch: Purity:99.77
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AZ5104 Chemical Structure
Chemical Structure
Check the AZ5104 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[2-[2-(dimethylamino)ethyl-methylamino]-5-[[4-(1H-indol-3-yl)pyrimidin-2-yl]amino]-4-methoxyphenyl]prop-2-enamide
CAS Number 1421373-98-9
Molecular Formula

C27H31N7O2
Molecular Weight (MW) 485.58
SMILES CN(C)CCN(C)C1=CC(=C(C=C1NC(=O)C=C)NC2=NC=CC(=N2)C3=CNC4=CC=CC=C43)OC
InChIKey IQNVEOMHJHBNHC-UHFFFAOYSA-N

Ordering Information

Biological Activity

AZ5104, a demethylated active metabolite of osimertinib, is a potent, irreversible epidermal growth factor receptor (EGFR) inhibitor targeting wild-type EGFR as well as activating and resistance mutants such as EGFR (L858R/T790M). By blocking EGFR autophosphorylation, AZ5104 inhibits downstream AKT and ERK signaling pathways, suppressing cellular proliferation and driving apoptosis in mutant EGFR non-small cell lung cancer models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 97 mg/mL (199.76 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 5 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.