CAS No. 366-93-8
Batch:
Purity:99.70
Chemical Structure
Check the
AY 9944
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | N-[(2-chlorophenyl)methyl]-1-[4-[[(2-chlorophenyl)methylamino]methyl]cyclohexyl]methanamine;dihydrochloride | |
|---|---|---|
| CAS Number | 366-93-8 | |
| Molecular Formula |
C22H28Cl2N2.2ClH |
|
| Molecular Weight (MW) | 464.30 | |
| InChIKey | NRVIEWRSGDDWHP-UHFFFAOYSA-N |
Biological Activity
AY 9944 is a potent cholesterol biosynthesis inhibitor targeting Δ7-sterol reductase with an IC50 of 13 nM. By blocking the conversion of 7-dehydrocholesterol to cholesterol, it disrupts distal sterol metabolic pathways, causing hypocholesterolemia and the cellular accumulation of precursor sterols. These perturbations alter cellular lipid homeostasis, rendering AY 9944 a valuable pharmacological model for studying Smith–Lemli–Opitz syndrome.[1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| Water | 21 mg/mL |
| DMSO |
8 mg/mL
(17.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 3 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.