CAS No. 86445-22-9
Batch:
Purity:99.91
Chemical Structure
Check the
Auxinole
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 4-(2,4-dimethylphenyl)-2-(1H-indol-3-yl)-4-oxobutanoic acid | |
|---|---|---|
| CAS Number | 86445-22-9 | |
| Molecular Formula |
C20H19NO3 |
|
| Molecular Weight (MW) | 321.37 | |
| SMILES | CC1=CC(=C(C=C1)C(=O)CC(C(O)=O)C2=C[NH]C3=C2C=CC=C3)C | |
| InChIKey | HGUYAIJBXSQXGV-UHFFFAOYSA-N |
Auxinole is a potent auxin antagonist and inhibitor of the transport inhibitor response 1 (TIR1) receptor. It binds to TIR1 and directly blocks the formation of the TIR1-IAA-Aux/IAA co-receptor complex. By preventing auxin-induced degradation mediated by TIR1/OsTIR1, auxinole suppresses the basal leaky degradation of degron-fused proteins, enabling tight control of targeted protein depletion in auxin-inducible degron systems. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
64 mg/mL
(199.14 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 5 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.