CAS No. 1216665-49-4

Batch: Purity:99.99
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APY29 Chemical Structure
Chemical Structure
Check the APY29 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-N-(3H-benzimidazol-5-yl)-4-N-(5-cyclopropyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine
CAS Number 1216665-49-4
Molecular Formula

C17H16N8
Molecular Weight (MW) 332.36
SMILES C1CC1C2=CC(=NN2)NC3=NC(=NC=C3)NC4=CC5=C(C=C4)N=CN5
InChIKey WJNBSTLIALIIEW-UHFFFAOYSA-N

Biological Activity

APY29 is a type I kinase inhibitor of IRE1α that binds to the ATP-binding site on IRE1α, inhibiting its autophosphorylation while allosterically activating its endoribonuclease function. By directly modulating IRE1α, APY29 regulates downstream unfolded protein response signaling pathways and XBP1 mRNA splicing, ultimately influencing endoplasmic reticulum stress responses, adaptive gene expression programs, and cell fate decisions under stress conditions. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 32 mg/mL (96.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Korennykh AV, et al. Nature. 2009;457(7230):687-93.

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Note: Technical data last updated: Sep 1, 2026.