CAS No. 916151-99-0
Batch:
Purity:98.20
Chemical Structure
Check the
APTO-253
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 2-(5-fluoro-2-methyl-1H-indol-3-yl)-1H-imidazo[4,5-f][1,10]phenanthroline | |
|---|---|---|
| CAS Number | 916151-99-0 | |
| Molecular Formula |
C22H14FN5 |
|
| Molecular Weight (MW) | 367.38 | |
| SMILES | CC1=C(C2=NC3=C([NH]2)C4=CC=CN=C4C5=C3C=CC=N5)C6=C([NH]1)C=CC(=C6)F | |
| InChIKey | NIRXBXIPHUTNNI-UHFFFAOYSA-N |
APTO-253 is a small-molecule inducer of KLF4 that selectively inhibits c-Myc expression and upregulates CDKN1A (p21). By enhancing KLF4 transcriptional activity, APTO-253 modulates the p21/c-Myc axis to suppress oncogenic signalling in acute myeloid leukemia cells. This regulatory response leads to G0-G1 cell-cycle arrest and triggers apoptotic cell death, thereby halting proliferation in leukemic cell populations. [1]
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Concentration
Volume
Mass
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|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
73 mg/mL
(198.7 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.