Choose Your Country or Region

Anti-Caldesmon/CDM Rabbit Antibody [N1A18]

Catalog No.: F2341

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:10000 - 1:20000 IP1:20 IHC1:100 IF1:50 FCM1:20

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
93 kDa 75 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human leiomyoma; Mouse kidney; Rat stomach; NIH/3T3
Negative Control	Rat liver; HEK-293

Datasheet & SDS

Biological Description

Specificity
Caldesmon/CDM Rabbit mAb detects endogenous levels of total Caldesmon/CDM protein.

Clone
N1A18

Synonym(s)
CAD, CDM, CALD1, Caldesmon

Background
Caldesmon is a protein present in both smooth muscle and non-muscle cells, existing in two main isoform classes generated through alternative splicing of a single gene. The high–molecular weight isoforms (89–93 kDa) are specific to adult, fully differentiated smooth muscle cells, whereas the low–molecular weight isoforms (59–63 kDa) are found in non-muscle cells and in de-differentiated smooth muscle cells. All isoforms share conserved regions responsible for caldesmon’s key functional properties, including binding to actin, tropomyosin, Ca²⁺–calmodulin, myosin, and phospholipids. They are also strong inhibitors of the actin–tropomyosin–activated myosin MgATPase. Despite these similarities, the smooth muscle and non-muscle isoforms have distinct physiological roles, reflected in their different cellular localizations. Non-muscle caldesmon functions as a regulator of the microfilament network, contributing to its assembly and stabilization. In contrast, smooth muscle caldesmon, in cooperation with tropomyosin, acts as a mediator of Ca²⁺-dependent inhibition of smooth muscle contraction.

Background

Caldesmon is a protein present in both smooth muscle and non-muscle cells, existing in two main isoform classes generated through alternative splicing of a single gene. The high–molecular weight isoforms (89–93 kDa) are specific to adult, fully differentiated smooth muscle cells, whereas the low–molecular weight isoforms (59–63 kDa) are found in non-muscle cells and in de-differentiated smooth muscle cells. All isoforms share conserved regions responsible for caldesmon’s key functional properties, including binding to actin, tropomyosin, Ca²⁺–calmodulin, myosin, and phospholipids. They are also strong inhibitors of the actin–tropomyosin–activated myosin MgATPase. Despite these similarities, the smooth muscle and non-muscle isoforms have distinct physiological roles, reflected in their different cellular localizations. Non-muscle caldesmon functions as a regulator of the microfilament network, contributing to its assembly and stabilization. In contrast, smooth muscle caldesmon, in cooperation with tropomyosin, acts as a mediator of Ca²⁺-dependent inhibition of smooth muscle contraction.

References
https://pubmed.ncbi.nlm.nih.gov/9415999/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%