CAS No. 51264-14-3
Batch:
Purity:97.29
Chemical Structure
Check the
Amsacrine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | N-[4-(acridin-9-ylamino)-3-methoxyphenyl]methanesulfonamide | |
|---|---|---|
| CAS Number | 51264-14-3 | |
| Molecular Formula |
C21H19N3O3S |
|
| Molecular Weight (MW) | 393.46 | |
| InChIKey | XCPGHVQEEXUHNC-UHFFFAOYSA-N |
Amsacrine (m-AMSA) is a DNA-intercalating antineoplastic agent that acts as a potent inhibitor of topoisomerase II. By intercalating into DNA and stabilizing the topoisomerase II-cleavable complex, amsacrine blocks DNA strand re-ligation, leading to double-strand DNA breaks and inhibition of DNA replication. These molecular perturbations disrupt DNA synthesis, trigger cell cycle arrest, and induce apoptotic cell death in cancer cells. [1][2]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
79 mg/mL
(200.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 2 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.