CAS No. 2417674-27-0
Batch:
Purity:96.76
Chemical Structure
Check the
AMPK-IN-3
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 2417674-27-0 | |
|---|---|---|
| Molecular Formula |
C25H33N5O3 |
|
| Molecular Weight (MW) | 451.56 |
AMPK-IN-3 is a potent oxindole-based ATP-site inhibitor of AMP-activated protein kinase (AMPK) that targets AMPK alpha subunit isoforms. By blocking AMPK activity, the compound suppresses downstream signaling pathways involved in cellular energy sensing and metabolic homeostasis. In chronic myeloid leukemia K562 cells, AMPK-IN-3 effectively suppresses intracellular AMPK activity without significantly reducing cell viability or inducing cytotoxicity. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
18 mg/mL
(39.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.