CAS No. 54-62-6
Batch:
Purity:98.83
Chemical Structure
Check the
Aminopterin
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid | |
|---|---|---|
| CAS Number | 54-62-6 | |
| Molecular Formula |
C19H20N8O5 |
|
| Molecular Weight (MW) | 440.41 | |
| SMILES | NC1=NC(=C2N=C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)C=NC2=N1)N | |
| InChIKey | TVZGACDUOSZQKY-LBPRGKRZSA-N |
Aminopterin is a folic acid antagonist and potent competitive inhibitor of tumor cell dihydrofolate reductase (DHFR). Inhibition of DHFR disrupts folate metabolism, suppressing both dTMP and de novo purine synthesis pathways. This downstream deprivation of nucleotide precursors leads to the inhibition of DNA synthesis and suppressed cellular proliferation, resulting in cell death. [1][2]
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Concentration
Volume
Mass
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|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
88 mg/mL
(199.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.