CAS No. 1416799-28-4

Batch: Purity:99.59
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AMG-333 Chemical Structure
Chemical Structure
Check the AMG-333 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 1416799-28-4
Molecular Formula

C20H12F5N3O4
Molecular Weight (MW) 453.32
SMILES C1=CC(=C(N=C1)C(C2=CC(=C(C=C2)OC(F)(F)F)F)NC(=O)C3=NC=C(C=C3)C(=O)O)F

Biological Activity

AMG-333 acts as a potent and selective antagonist targeting human and rat transient receptor potential melastatin 8 (TRPM8) ion channels. By blocking TRPM8 channel activation, AMG-333 inhibits menthol- and cold-induced calcium influx in primary sensory neurons, suppressing downstream thermal somatosensation and sensory signaling pathways. Blockade of this channel reduces cold allodynia and attenuates neuropathic pain responses in preclinical models. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 91 mg/mL (200.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 91 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] McKemy DD, et al. Nature. 2002;416(6876):52-8.

[2] Colburn RW, et al. Neuron. 2007;54(3):379-86.

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Note: Technical data last updated: Sep 1, 2026.