CAS No. 1357350-60-7
Batch:
Purity:98.69
Chemical Structure
Check the
ALLO-2
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 1357350-60-7 | |
|---|---|---|
| Molecular Formula |
C18H12F3N5O |
|
| Molecular Weight (MW) | 371.32 | |
| SMILES | FC(F)(F)OC1=CC=C(C=C1)C2=NC(=NC=C2)NC3=CC4=C([NH]N=C4)C=C3 |
ALLO-2 is a potent antagonist targeting both wild-type mouse Smoothened (Smo) and the drug-resistant DH mutant human Smo. By binding Smo at a distinct site, it effectively blocks agonist-stimulated Gli reporter luciferase activity in TM-Gli-Luc cells. This suppression of the Hedgehog/GLI signaling pathway inhibits downstream Gli-mediated gene transcription, thereby blunting oncogenic signals and overcoming treatment resistance in Hedgehog-dependent cell models. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
74 mg/mL
(199.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 5 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.