CAS No. 2364554-48-1

Batch: Purity:99.98
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Aficamten (CK-274) Chemical Structure
Chemical Structure
Check the Aficamten (CK-274) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[(1R)-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-2,3-dihydro-1H-inden-1-yl]-1-methylpyrazole-4-carboxamide
CAS Number 2364554-48-1
Molecular Formula

C18H19N5O2
Molecular Weight (MW) 337.38
SMILES CCC1=NC(=NO1)C2=CC=C3C(CCC3=C2)NC(=O)C4=C[N](C)N=C4
InChIKey IOVAZWDIRCRMTM-OAHLLOKOSA-N

Ordering Information

Biological Activity

Aficamten (CK-274) is a selective small-molecule inhibitor of cardiac myosin. It diminishes myosin ATPase activity by slowing phosphate release and stabilizing a weak actin-binding state, thereby preventing entry into the force-generating state. This action reduces the number of active actin-myosin cross-bridges and suppresses sarcomere shortening, ultimately decreasing myocardial contractility in cardiac myocytes and hypertrophic models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 67 mg/mL (198.58 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 15 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.