CAS No. 439081-18-2
Batch:
Purity:99.44
Chemical Structure
Check the
(E/Z)-Afatinib
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (E)-N-[4-(3-chloro-4-fluoroanilino)-7-[(3S)-oxolan-3-yl]oxyquinazolin-6-yl]-4-(dimethylamino)but-2-enamide | |
|---|---|---|
| CAS Number | 439081-18-2 | |
| Molecular Formula |
C24H25ClFN5O3 |
|
| Molecular Weight (MW) | 485.94 | |
| InChIKey | ULXXDDBFHOBEHA-CWDCEQMOSA-N |
(E/Z)-Afatinib is an irreversible second-generation tyrosine kinase inhibitor targeting wild-type EGFR, EGFR L858R, HER2, and ErbB4 (IC50 1 nM). By covalently binding to the kinase domains of ErbB family receptors, it blocks downstream RAS/MAPK and PI3K/AKT signaling pathways. Consequently, afatinib suppresses cellular proliferation and induces apoptosis in ErbB-dependent carcinoma models. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
97 mg/mL
(199.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 3 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.