CAS No. 252025-52-8

Batch: Purity:98.61
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Adjudin Chemical Structure
Chemical Structure
Check the Adjudin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2,4-dichlorophenyl)methyl]indazole-3-carbohydrazide
CAS Number 252025-52-8
Molecular Formula

C15H12Cl2N4O
Molecular Weight (MW) 335.19
SMILES C1=CC=C2C(=C1)C(=NN2CC3=C(C=C(C=C3)Cl)Cl)C(=O)NN
InChIKey VENCPJAAXKBIJD-UHFFFAOYSA-N

Ordering Information

Biological Activity

Adjudin (AF-2364) is a male contraceptive agent and blocker of chloride channels. It displays anti-inflammatory properties by suppressing NF-kB p65 nuclear translocation, DNA-binding activity, and ERK/MAPK phosphorylation. Downstream, these molecular actions suppress pro-inflammatory signaling and disrupt Sertoli-germ cell adherens junctions, leading to reversible germ cell loss from the seminiferous epithelium. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 67 mg/mL (199.88 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 2 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.