CAS No. 1866-15-5

Batch: Purity:99.00
Acetylthiocholine iodide Chemical Structure
Chemical Structure
Check the Acetylthiocholine iodide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 1866-15-5
Molecular Formula

C7H16NOS.I

Molecular Weight (MW) 289.18

Ordering Information

Biological Activity

Acetylthiocholine iodide acts as a chromogenic synthetic substrate for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Enzymatic cleavage of acetylthiocholine iodide releases free thiocholine, which reacts with thiol reagents to enable colorimetric quantification of cholinesterase activity. This process regulates functional readout of cholinergic neurotransmission pathways and serves as an essential tool to investigate AChE inhibition, organophosphate neurotoxicity, and central nervous system disorders.

How to Prepare Stock Solution?

Concentration Volume Mass
1 mg 5 mg 10 mg
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 58 mg/mL (200.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 58 mg/mL
Ethanol 4 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.