CAS No. 30931-67-0
Batch:
Purity:99.75
Chemical Structure
Check the
ABTS
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | azane;(2Z)-3-ethyl-2-[(Z)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid | |
|---|---|---|
| CAS Number | 30931-67-0 | |
| Molecular Formula |
C18H18N4O6S4.2H3N |
|
| Molecular Weight (MW) | 548.68 | |
| InChIKey | OHDRQQURAXLVGJ-AXMZSLBLSA-N |
Biological Activity
ABTS (ABTS diammonium salt) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates and peroxidase enzymes. Upon oxidation by peroxidase activity or free radicals, ABTS generates a stable, green-colored radical cation (ABTS•+) with distinct spectrophotometric absorbance. This oxidation reaction is widely applied to evaluate total antioxidant capacity (TAC) and free radical scavenging activity in biological fluids via the Trolox equivalent antioxidant capacity (TEAC) assay.[1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
17 mg/mL
(30.98 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 13 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.