CAS No. 2070009-66-2

Batch: Purity:99.66
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A-674563 HCl Chemical Structure
Chemical Structure
Check the A-674563 HCl product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-1-[[5-(3-methyl-2H-indazol-5-yl)-3-pyridinyl]oxy]-3-phenylpropan-2-amine;hydrochloride
CAS Number 2070009-66-2
Molecular Formula

C22H22N4O.CIH
Molecular Weight (MW) 394.9
SMILES CC1=C2C=C(C=CC2=NN1)C3=CC(=CN=C3)OCC(CC4=CC=CC=C4)N
InChIKey HLNHYVLLEFHBJD-FYZYNONXSA-N

Biological Activity

A-674563 HCl is a potent, selective Akt inhibitor with an IC50 of 14 nM (Ki of 11 nM for Akt1) that also inhibits PKA (IC50 16 nM) and CDK2 (IC50 46 nM). By targeting the AKT/mTOR signaling pathway, it inhibits tumor cell proliferation and suppresses downstream survival signals. In vivo, A-674563 demonstrates antitumor efficacy and enhances the therapeutic efficacy of paclitaxel in PC-3 xenograft models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 72 mg/mL (182.32 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 72 mg/mL
Ethanol 18 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] J. Med. Chem. 2006;49(8):2668-2668.

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Note: Technical data last updated: Sep 1, 2026.