CAS No. 70-00-8

Batch: Purity:99.96
Check CoA
Trifluridine (NSC 75520) Chemical Structure
Chemical Structure
Check the Trifluridine (NSC 75520) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-(trifluoromethyl)pyrimidine-2,4-dione
CAS Number 70-00-8
Molecular Formula

C10H11F3N2O5
Molecular Weight (MW) 296.2
SMILES C1C(C(OC1N2C=C(C(=O)NC2=O)C(F)(F)F)CO)O
InChIKey VSQQQLOSPVPRAZ-RRKCRQDMSA-N

Ordering Information

Biological Activity

Trifluridine (NSC 75520) is a nucleoside analogue antiviral and antineoplastic agent that targets viral and cellular DNA replication. Upon intracellular phosphorylation to its monophosphate and triphosphate forms, it inhibits thymidylate synthase and incorporates directly into DNA strands, disrupting base pairing and DNA structure. This incorporation induces double-strand DNA breaks and inhibits viral DNA synthesis, leading to suppression of viral replication and cell proliferation. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 59 mg/mL (199.18 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 59 mg/mL
Ethanol 59 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.