Clinical Trials

Teniposide has been evaluated across Phase 1 through Phase 4 clinical trials, investigating its efficacy as a monotherapy or within multi-agent chemoimmunotherapy regimens for conditions including acute lymphoblastic leukemia, primary central nervous system lymphoma, mantle cell lymphoma, and extensive-stage small-cell lung cancer. Sponsored by academic medical centers and research foundations, trial recruitment statuses range from completed historical studies to active, recruiting protocols testing modern combination approaches for hematologic and solid tumors.

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT07074470 RECRUITING
Primary Central Nervous System Lymphoma (PCNSL)
The First Affiliated Hospital of Soochow University
2025-01-02
NCT07185373 RECRUITING
Primary Central Nervous System Lymphoma
Huashan Hospital
2025-10-01 PHASE2; PHASE3
NCT07583511 RECRUITING
Small-cell Lung Cancer
Sun Yat-sen University
2024-02-01 PHASE2
NCT07188441 RECRUITING
GCT; Germ Cell Tumor; Central Malignant Germ Cell Tumor
Chengcheng Guo
2024-08-13 PHASE2
NCT06758700 RECRUITING
Extensive-stage Small Cell Lung Cancer (ES-SCLC); Thoracic Neoplasms; Lung Neoplasms; Lung Diseases
Shanghai Pulmonary Hospital, Shanghai, China
2025-02-07 PHASE2
NCT06048107 UNKNOWN
HLH
Beijing Friendship Hospital
2023-10-22
NCT01700946 COMPLETED
Recurrent B-Cell Childhood Acute Lymphoblastic Leukemia; Recurrent Childhood B-Lymphoblastic Lymphoma
St. Jude Children's Research Hospital
2013-04-15 PHASE2
NCT00186875 COMPLETED
Acute Lymphoblastic Leukemia; Lymphoma, Lymphoblastic
St. Jude Children's Research Hospital
2003-11 PHASE2
NCT00853008 COMPLETED
Acute Lymphoblastic Leukemia
PETHEMA Foundation
2003-01 PHASE4
NCT00198991 COMPLETED
Adult Acute Lymphocytic Leukemia
Goethe University
2003-04 PHASE4
NCT00004231 COMPLETED
Lymphoma
Northwestern University
1999-10 PHASE2
NCT00187083 COMPLETED
Acute Lymphoblastic Leukemia
St. Jude Children's Research Hospital
1997-02 PHASE3
NCT00004916 COMPLETED
Lymphoma
Northwestern University
1999-02 PHASE1; PHASE2
NCT00002531 UNKNOWN
Leukemia
Johann Wolfgang Goethe University Hospital
1993-01 PHASE2
NCT00343369 UNKNOWN
Leukemia
Universitätsklinikum Hamburg-Eppendorf
2003-01

(data from https://clinicaltrials.gov, updated on 2025-07-20)

Check the Teniposide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Compliance for Clinical Use

Mechanism and Biochemical Profile

Teniposide inhibits topoisomerase II activity without intercalating into DNA, causing dose-dependent single- and double-stranded DNA breaks that impair DNA replication and trigger apoptotic cell death in neoplastic cells. This inhibition of topoisomerase-mediated DNA repair blocks tumor cell proliferation, serving as the biological rationale for its clinical trial evaluation in acute lymphoblastic leukemia, primary central nervous system lymphoma, and small-cell lung cancer.

Appendix RUO and cGMP Quality Standards

Quality Dimension RUO (Research Use Only) cGMP (Current Good Manufacturing Practice)
Clinical Applicability Prohibited in human clinical trials or medical diagnostics. Mandatory for human clinical trials (Phase I–III) and therapies.
Regulatory Status Non-regulated grade; exempt from drug manufacturing laws. Legally enforced by health authorities (e.g., FDA, EMA, NMPA).
Facility Environment Unclassified analytical or research laboratories. Validated Cleanrooms (ISO Class 5–8) with continuous monitoring.
Quality Control Basic purity and activity testing. Rigorous safety release testing (Sterility, Endotoxin, Mycoplasma).
Process Validation Basic equipment calibration; no process validation required. Full qualification (IQ/OQ/PQ) and complete batch records.
Quality Assurance Vendor self-declared without required formal QMS. Mandatory QA/QC unit, Change Control, CAPA, and vendor audits.
Regulatory Impact High risk of IND rejection if used as a critical raw material. Required for IND/NDA filings, supported by Drug Master Files (DMF).

Footnotes

Regulatory Note: Governed by FDA (21 CFR Parts 210/211/312), EMA (EudraLex Vol 4), ICH Guidelines (E6/Q7/Q9/Q10), and compendial standards (USP <71>/<85>/<1043>).

Note: Technical data last updated: Sep 1, 2026.