CAS No. 163253-35-8

Batch: Purity:99.98
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Sitafloxacin Hydrate Chemical Structure
Chemical Structure
Check the Sitafloxacin Hydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 7-[(7S)-7-amino-5-azaspiro[2.4]heptan-5-yl]-8-chloro-6-fluoro-1-[(1R,2S)-2-fluorocyclopropyl]-4-oxoquinoline-3-carboxylic acid
CAS Number 163253-35-8
Molecular Formula

C19H18ClF2N3O3.3/2H2O
Molecular Weight (MW) 436.84
SMILES C1CC12CN(CC2N)C3=C(C=C4C(=C3Cl)N(C=C(C4=O)C(=O)O)C5CC5F)F.O
InChIKey PNUZDKCDAWUEGK-CYZMBNFOSA-N

Biological Activity

Sitafloxacin Hydrate is a broad-spectrum oral fluoroquinolone antibiotic that targets bacterial DNA gyrase and topoisomerase IV. By inhibiting these essential enzymes, sitafloxacin disrupts bacterial DNA replication, transcription, and repair, leading to rapid bactericidal cell death. It exhibits potent antibacterial activity against a wide variety of Gram-positive, Gram-negative, and anaerobic clinical isolates, including strains resistant to other fluoroquinolones. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 15 mg/mL (34.33 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Keating GM. Drugs. 2011;71(18):2511-29.

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Note: Technical data last updated: Sep 1, 2026.