CAS No. 72559-06-9

Batch: Purity:99.91
Rifabutin (LM427) Chemical Structure
Chemical Structure
Check the Rifabutin (LM427) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name [2,15,17,32-tetrahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-1'-(2-methylpropyl)-6,23-dioxospiro[8,33-dioxa-24,27,29-triazapentacyclo[23.6.1.14,7.05,31.026,30]tritriaconta-1(31),2,4,9,19,21,25(32),26,29-nonaene-28,4'-piperidine]-13-yl] acetate
CAS Number 72559-06-9
Molecular Formula

C46H62N4O11

Molecular Weight (MW) 847
SMILES CC1C=CC=C(C(=O)N=C2C(=C3C(=C4C2=NC5(N4)CCN(CC5)CC(C)C)C6=C(C(=C3O)C)OC(C6=O)(OC=CC(C(C(C(C(C(C1O)C)O)C)OC(=O)C)C)OC)C)O)C
InChIKey AZFBLLCNOQPJGJ-UHFFFAOYSA-N

Ordering Information

Biological Activity

Rifabutin (LM427) is a semisynthetic ansamycin antibiotic that acts as a potent inhibitor of bacterial DNA-dependent RNA polymerase. By binding to bacterial RNA polymerase, it suppresses the initiation of RNA chain formation, resulting in the inhibition of RNA transcription and synthesis. This suppression of transcription disrupts bacterial protein expression, leading to potent antimycobacterial activity against Mycobacterium species. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
1 mg 5 mg 10 mg
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (118.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.