CAS No. 57-63-6

Batch: Purity:99.85
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Ethinyl Estradiol Chemical Structure
Chemical Structure
Check the Ethinyl Estradiol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 57-63-6
Molecular Formula

C20H24O2
Molecular Weight (MW) 296.4
SMILES CC12CCC3C(C1CCC2(C#C)O)CCC4=C3C=CC(=C4)O

Ordering Information

Biological Activity

Ethinyl Estradiol is an orally bioactive synthetic estrogen that acts as a potent agonist of nuclear estrogen receptors, including ERα and ERβ. Binding of Ethinyl Estradiol to these receptors induces conformational changes, receptor dimerization, and translocation to the nucleus to regulate gene transcription. This downstream transcriptional regulation modulates signaling pathways governing cellular proliferation, endocrine signaling, and physiological homeostasis in estrogen-responsive tissues. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 59 mg/mL (199.05 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 59 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.