CAS No. 4291-63-8

Batch: Purity:99.99
Cladribine Chemical Structure
Chemical Structure
Check the Cladribine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2R,3S,5R)-5-(6-amino-2-chloropurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol
CAS Number 4291-63-8
Molecular Formula

C10H12ClN5O3

Molecular Weight (MW) 285.69
SMILES C1C(C(OC1N2C=NC3=C(N=C(N=C32)Cl)N)CO)O
InChIKey PTOAARAWEBMLNO-KVQBGUIXSA-N

Ordering Information

Biological Activity

Cladribine is a synthetic purine nucleoside analog and antimetabolite that targets proliferating and resting lymphocytes. Upon intracellular phosphorylation into its active triphosphate derivative, cladribine inhibits DNA synthesis and ribonucleotide reductase activity, inducing DNA strand breaks and activating apoptotic pathways. This mechanistically drives sustained cytotoxicity across hematologic cell lines, including multiple myeloma models such as U266, RPMI8226, and MM1.S. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 57 mg/mL (199.51 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.

Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.

Note: Technical data last updated: Sep 1, 2026.