CAS No. 481-74-3

Batch: Purity:99.77
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Chrysophanic Acid Chemical Structure
Chemical Structure
Check the Chrysophanic Acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1,8-dihydroxy-3-methylanthracene-9,10-dione
CAS Number 481-74-3
Molecular Formula

C15H10O4
Molecular Weight (MW) 254.24
SMILES CC1=CC2=C(C(=C1)O)C(=O)C3=C(C2=O)C=CC=C3O
InChIKey LQGUBLBATBMXHT-UHFFFAOYSA-N

Ordering Information

Biological Activity

Chrysophanic acid (Chrysophanol) is a natural anthraquinone inhibitor targeting the EGFR/mTOR signaling pathway. In EGFR-overexpressing SNU-C5 human colon cancer cells, it selectively suppresses cell proliferation without affecting cell lines with low EGFR expression. Mechanistically, Chrysophanic acid attenuates EGF-induced EGFR phosphorylation and blocks downstream signaling molecules, including AKT, ERK, and mTOR/p70S6K, demonstrating enhanced antiproliferative activity when combined with rapamycin.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 8 mg/mL (31.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.