CAS No. 474-25-9

Batch: Purity:100.00
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Chenodeoxycholic Acid Chemical Structure
Chemical Structure
Check the Chenodeoxycholic Acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (4R)-4-[(3R,5S,7R,8R,9S,10S,13R,14S,17R)-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid
CAS Number 474-25-9
Molecular Formula

C24H40O4
Molecular Weight (MW) 392.57
SMILES CC(CCC(=O)O)C1CCC2C1(CCC3C2C(CC4C3(CCC(C4)O)C)O)C
InChIKey RUDATBOHQWOJDD-BSWAIDMHSA-N

Ordering Information

Biological Activity

Chenodeoxycholic Acid is a primary bile acid and endogenous agonist of the farnesoid X receptor (FXR). Activation of FXR by chenodeoxycholic acid regulates gene expression involved in bile acid synthesis, transport, and cholesterol metabolism. Downstream activation of FXR signaling modulates lipid homeostasis, represses hepatic cholesterol 7α-hydroxylase expression, and suppresses endogenous bile acid production. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 79 mg/mL (201.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 79 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.